Thermo Fisher Scientific LXR alpha Recombinant Rabbit Monoclonal Antibody (JA20-38)
LXR alpha 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합. 높은 특이성과 재현성을 제공하며, 동물 유래 성분이 없는 포뮬레이션. 장기 보관 시 -20°C에서 안정적 유지.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA20-38 |
| Immunogen | Synthetic peptide within Human LXR alpha (aa 49–98) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809968 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
NR1H3 (oxysterols receptor LXR-alpha) is a nuclear receptor with ligand-dependent transcriptional activation activity. It interacts with retinoic acid receptor (RXR), converting RXR into an active ligand-binding subunit that mediates retinoid responses via LXRES target genes.
LXRES are DR4-type response elements characterized by direct repeats of two similar hexanucleotide half-sites spaced by four nucleotides.
NR1H3 plays a key role in cholesterol homeostasis by regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR, and LRP8, and functionally interacts with RORA to regulate genes involved in liver metabolism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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