Thermo Fisher Scientific Clathrin Heavy Chain Recombinant Rabbit Monoclonal Antibody (JA53-02)
Recombinant rabbit monoclonal antibody recognizing Clathrin Heavy Chain. Validated for WB, IHC(P), ICC/IF. Offers high specificity, sensitivity, and lot-to-lot consistency. Suitable for research use in human, mouse, rat, and primate samples.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA53-02 |
| Immunogen | Synthetic peptide within Human Clathrin heavy chain aa 201–250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809967 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed, followed by screening to select optimal clones.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
Clathrin is the major protein component of the polyhedral coat of coated pits and vesicles. It plays a key role in vesicle formation, with adapter protein complexes linking the clathrin lattice to the plasma membrane or trans-Golgi network.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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