Thermo Fisher Scientific Phospho-Mdm2 (Tyr394) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

This antibody detects endogenous levels of MDM2 only when phosphorylated at Tyr394.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in regulating the stability of p53.
It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, facilitating MDM2-mediated ubiquitination and degradation of p53.
Phosphorylation of MDM2 blocks its binding to p19ARF, enhancing p53 degradation.
MDM2 negatively regulates p53 function by binding and inhibiting its transactivation role. Overexpression of MDM2 can inactivate p53, reducing its tumor suppressor activity.
MDM2 also possesses E3 ubiquitin ligase activity, contributing to p53 degradation.
Additionally, MDM2 plays roles in apoptosis and cell cycle regulation and is overexpressed in various human malignancies, including soft tissue carcinomas and breast cancer.
Beyond p53, MDM2 interacts with proteins such as retinoblastoma 1 and ribosomal protein L5, influencing cell cycle, apoptosis, and tumorigenesis.
More than 40 alternatively spliced transcript variants of MDM2 have been identified in both tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.