Thermo Fisher Scientific CD150 Monoclonal Antibody (A12 (7D4)), PE, eBioscience

Thermo Fisher Scientific CD150 Monoclonal Antibody (A12 (7D4)), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 2 publications

Product Specifications

Product Specific Information

The A12 monoclonal antibody reacts with human CD150, an ~70 kDa transmembrane glycoprotein known as Signaling Lymphocyte Activation Molecule (SLAM).
CD150 is expressed by T and B cells and is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.
Signaling through SLAM induces proliferation and cytokine production of T cells and plays a role in activation of T and B cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
Recommended Use: 5 µL (0.5 µg) per test (final volume 100 µL, 10⁵–10⁸ cells/test)
Excitation/Emission: 488–561 nm / 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation.
It is expressed on memory T cells, T cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation.
Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM.
SLAM-SLAM interactions enhance expansion and differentiation of activated B cells.
CD150 acts as a receptor for measles virus and co-activator on T and B cells.
It interacts with SH2D1A and PTPN11 via its cytoplasmic domain.
Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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