Thermo Fisher Scientific CD146 Monoclonal Antibody (P1H12), PE, eBioscience
CD146 단백질을 인식하는 P1H12 클론 단일클론 항체로, PE 형광체가 결합된 제품입니다. 인간 및 마우스 등 다양한 종에서 반응하며, 유세포 분석(Flow Cytometry)에 적합합니다. 고순도 정제 및 안정적인 형광 특성을 제공합니다.
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Thermo Fisher Scientific CD146 Monoclonal Antibody (P1H12), PE, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 23 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rabbit |
| Published Species | Human, Mouse, Rabbit |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | P1H12 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11042581 |
Additional Formats
- Unconjugated
- eFluor 450
- eFluor 660
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Yellow 730
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The monoclonal antibody P1H12 recognizes CD146, also known as MUC18, s-endo, Endo-CAM, and Mel-CAM, a member of the Ig superfamily.
CD146 is expressed on endothelial cells, bone marrow fibroblasts, some tumors (especially melanoma), mesenchymal stromal cells, and endometrial stromal cells.
Expression on circulating blood cells is limited to a subset of T cells rather than endothelial cells. The protein is heavily glycosylated, with over 50% of its mass derived from carbohydrates.
- Cross-reactivity: Mouse, Rabbit, Canine (not Rat)
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested on normal human peripheral blood cells
- Recommended Use: 5 µL (0.125 µg) per test in 100 µL final volume
- Excitation: 488–561 nm
- Emission: 578 nm
- Laser Compatibility: Blue, Green, Yellow-Green
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD146 (MCAM) is an integral membrane glycoprotein of the immunoglobulin superfamily.
It is expressed at cell-cell junctions in endothelial cells and various normal and malignant cell types.
CD146 functions as a calcium-independent adhesion molecule and signal transduction mediator, recruiting Fyn kinase and promoting tyrosine-phosphorylation of proteins involved in actin cytoskeleton assembly.
It plays a key role in endothelial cohesion, melanoma metastasis, and neural crest cell adhesion during embryonic development.
CD146 also acts as a surface receptor that triggers phosphorylation of FYN and PTK2, and transiently increases intracellular calcium concentration.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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