Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), PE, eBioscience

Thermo Fisher Scientific CD150 Monoclonal Antibody (9D1), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 7 publications

Product Specifications

Additional Formats:

• APC
• Biotin
• FITC
• Functional Grade
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The 9D1 monoclonal antibody reacts with mouse CD150, an ~70 kDa transmembrane glycoprotein known as Signaling Lymphocyte Activation Molecule (SLAM).
CD150 is expressed by T (especially TH1) and B cells and is rapidly upregulated upon activation. Immature thymocytes and dendritic cells also express this antigen.
Signaling through SLAM in T cells induces proliferation and enhances interferon-gamma response. SLAM contributes to adhesion between T cells and antigen-presenting cells. 9D1 is reported to be an activating antibody.

Mouse hematopoietic stem cells (HSC) can be identified using SLAM family markers such as CD150⁺CD244⁻CD48⁻. For this application, antibody clone mShad150 (Product #12-1502) is recommended.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse thymic and splenic cells.
Use ≤ 0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Optimize antibody titration for best assay performance.

Excitation: 488–561 nm
Emission: 578 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD150 (SLAM, Signaling Lymphocyte Activation Molecule) belongs to the immunoglobulin gene superfamily and is involved in T cell stimulation.
SLAM is expressed on peripheral blood memory T cells, immature thymocytes, and B cells, and is rapidly induced upon activation.
Activated B cells express membrane-bound, soluble, and cytoplasmic isoforms of SLAM.
Homophilic SLAM-SLAM interactions during B–B and B–T cell contact enhance activated B cell expansion and differentiation.
The extracellular domain of CD150 serves as the measles virus receptor and co-activator on T/B cells.
CD150 interacts with SH2D1A and PTPN11 via its cytoplasmic domain.
Mutations in the CD150 gene may be associated with X-linked lymphoproliferative disease (XLP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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