Thermo Fisher Scientific Histone H2B Monoclonal Antibody (C.362.2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

ChIP assay (ChIP)

1.5 µg/1x10^6 cells

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat, Zebrafish

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C.362.2

Immunogen

Synthetic peptide corresponding to the carboxy-terminal residues of histone H2B

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

12 mg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10982286

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other histones.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.