Thermo Fisher Scientific Podoplanin Monoclonal Antibody (D2-40)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:40

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:40

-

Product Specifications

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

D2-40

Immunogen

Resected tissue from dysgeminoma of the ovary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Storage conditions

4°C or -20°C if preferred

Shipping conditions

Wet ice

RRID

AB_2162066

Product Specific Information

A suggested positive control for immunohistochemical applications is human lymph node. This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.

Mouse anti human podoplanin antibody, clone D2-40 was raised against M2A antigen (Marks et al, 1999) and detects podoplanin (Sonne et al, 2006).

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin type-1 glycoprotein with a 40-43 kDa molecular weight. Podoplanin is expressed in many tumors and normal cells, especially lymphatic epithelial cells and follicular DCs. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. Podoplanin appears to serve as a ligand for CLEC-2 and expression is positively correlated with tumors expressing greater invasive and metastatic potential. Podoplanin is directly involved in cell migration, aids metastases formation and tumor cell invasion of tissue. Further, it has also been determined that podoplanin expression is induced through Src activation of Cas and, recently, there are indications of podoplanin presence on Th17 cells. Initial studies below indicate the presence of podoplanin on a small but distinct population of CD4+ cells from human PBMC. Dysfunction of the podoplanin protein has been linked to diseases such as lymphangioma and subglottis neoplasm. Podoplanin has also been proposed as a marker for lung injury due to the physiological function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.