Thermo Fisher Scientific Podoplanin Monoclonal Antibody (D2-40)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Suggested positive control for immunohistochemical applications: human lymph node
• No protein digestion pre-treatment required for paraffin embedded sections
• No heat antigen retrieval required prior to staining of paraffin embedded sections
• Mouse anti-human podoplanin antibody, clone D2-40, was raised against M2A antigen (Marks et al., 1999) and detects podoplanin (Sonne et al., 2006)

Target Information

Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin-type 1 glycoprotein with a molecular weight of 40–43 kDa. It is expressed in various tumors and normal cells, particularly lymphatic epithelial cells and follicular dendritic cells. Podoplanin localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells.
As a regulator of the lymphatic endothelium, podoplanin plays a role in maintaining the shape of podocytes and serves as a ligand for CLEC-2. Its expression correlates with tumor invasiveness and metastatic potential. Podoplanin is involved in cell migration, metastasis formation, and tumor invasion.
Expression can be induced via Src activation of Cas, and recent studies suggest its presence on Th17 cells. It has been detected on a small population of CD4+ cells from human PBMC. Dysfunction of podoplanin is linked to diseases such as lymphangioma and subglottis neoplasm. It is also proposed as a marker for lung injury due to its physiological role.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.