Thermo Fisher Scientific CD8a Monoclonal Antibody (OKT8 (OKT-8)), Alexa Fluor 488, eBioscience
인간 CD8a 인식 단클론 항체로, Alexa Fluor 488로 표지되어 세포분석(Flow Cytometry)에 적합합니다. OKT8 클론으로 CD8αβ 및 CD8αα 복합체를 인식하며, T세포 활성화 연구에 유용합니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 22 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT8 (OKT-8) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10548030 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, APC-eFluor 780
- Biotin, eFluor 450, FITC
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32–34 kDa cell surface receptor expressed as a heterodimer (CD8αβ) or homodimer (CD8αα). It is expressed on most thymocytes and subsets of mature T cells and NK cells. CD8 binds MHC class I and associates with protein tyrosine kinase p56lck, playing a role in T-cell development and activation.
Preliminary testing shows that OKT8 does not compete with clones RPA-T8 or HIT8a for binding to human peripheral blood leukocytes, indicating distinct epitopes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells.
Use at 20 µL (0.06 µg)/test. A test is defined as the amount of antibody staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction and T cell differentiation. It functions as a co-receptor with TCR for MHC-I molecules and is primarily expressed on cytotoxic T cells.
CD8α chain is essential for MHC-I binding and also expressed on subsets of NK cells, monocytes, and dendritic cells. Engagement with MHC-I/peptide complexes triggers Lck recruitment, leading to cytokine production and CTL activation. Activated CTLs are key in pathogen and tumor clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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