Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV Series (395–810)
• Brilliant Violet Series (480–800)
• eFluor Series (450–660)
• FITC
• Functional Grade
• NovaFluor Series (Blue, Red, Violet, Yellow; 510–810)
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436–780)
• Custom conjugation available upon request

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells, playing a critical role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck via its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis.
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume).
• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser
• Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen mediates recognition of MHC class II molecules and acts as a co-receptor for HIV. CD4 is mainly expressed on T helper cells but also found on monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of the brain and gut. CD4 participates in early T-cell activation via interaction with Lck and may contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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