Thermo Fisher Scientific SOX2 Polyclonal Antibody
SOX2 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 인간, 생쥐, 랫드 등에서 반응. 줄기세포 유지 및 신경 발달 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SOX2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.15 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792291 |
Product Specific Information
- Keep as a concentrated solution.
- Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (92%), Japanese Medaka (88%), Xenopus laevis (90%), Cat (100%), Pig (98%), Chicken (94%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%).
- Positive control: NT2D1, hESC, Mouse ESC.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in embryonic development and cell fate determination. The SOX2 protein is essential for stem-cell maintenance in the central nervous system and regulates gene expression in the stomach. It lies within an intron of another gene, SOX2 overlapping transcript (SOX2OT). SOX2 may act as a transcriptional activator through complex formation with other proteins. Mutations in SOX2 are associated with bilateral anophthalmia, optic nerve hypoplasia, and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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