Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), Biotin, eBioscience
마우스 CCR7(CD197)을 인식하는 4B12 클론의 비오틴 결합 단일클론 항체입니다. Flow Cytometry와 ICC/IF에 적합하며, 37°C에서 향상된 염색 강도를 제공합니다. 면역세포 이동 및 림프구 분화 연구에 활용됩니다. Thermo Fisher eBioscience 제품입니다.
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Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (4B12), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 33 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4B12 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_466641 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The 4B12 monoclonal antibody reacts with mouse CCR7 (CD197), also known as EBI-1. CCR7 is a chemokine receptor for CCL19 (ELC, MIP3β) and CCL21 (SLC, MIP2β), mediating lymphocyte migration to secondary lymphoid organs (SLO). It regulates T cell trafficking, dendritic cell localization, and B cell positioning during immune responses.
Human CCR7 is a marker for central memory T cells (TCM) and plays a role in thymocyte development. Deficiency leads to altered thymic architecture and impaired T cell maturation.
For optimal visualization, staining at 37°C yields brighter signals and better resolution than 2–8°C. Co-staining with other antigens is generally unaffected but should be validated experimentally.
Recommended Staining Protocol
- Prepare cell suspension and block FcγIII/II receptors using 5 µg/million cells anti-mouse CD16/32 for 15 min on ice.
- Add 1 µg/million cells of 4B12 antibody and incubate at 37°C or 2–8°C for 30 min.
- Wash once with Flow Cytometry Staining Buffer and decant.
- Analyze by flow cytometry or proceed with secondary staining.
Note: Co-staining with CCR7 ligand CCL19-Fc may be hindered due to steric interference. 4B12 binding can block CCL19-Fc detection, but not vice versa.
Target Information
CCR7 is a G protein-coupled receptor induced by Epstein-Barr virus (EBV) and expressed in blood, bone marrow, lymph node, and intestine. It regulates migration of memory T cells and dendritic cell maturation. Ligands include CCL19 (ECL) and CCL21. CCR family members (CCR1–CCR5) play key roles in T cell chemotaxis and phagocyte migration to inflammation sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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