Thermo Fisher Scientific Bax Monoclonal Antibody (1D1), eBioscience
Rat Bax 단백질에 특이적인 단클론 항체로, Western blot 및 면역침강(IP)에 사용 가능. Bcl-2나 Bcl-xL과 교차 반응 없음. 세포사멸 연구 및 단백질 발현 분석에 적합. 0.5 mg/mL 농도의 액상 항체로 4°C 보관.
- 판매단위
- pk
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 2 publications |
| Immunoprecipitation (IP) | 1–10 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468393 |
Product Specific Information
Description:
The 1D1 monoclonal antibody reacts with rat Bax (~21 kDa) and does not react with human or mouse Bax. It shows no cross-reactivity with Bcl-2 or Bcl-xL proteins. Bcl-2, Bax, and Bcl-xL are members of a protein family that regulates apoptosis. While Bcl-2 and Bcl-xL inhibit apoptosis, overexpression of Bax promotes programmed cell death. The immunogen was a rat Bax peptide sequence corresponding to amino acids 3–16.
Applications Reported:
The 1D1 antibody is reported for use in immunoprecipitation and Western blotting (WB).
Applications Tested:
- Western Blot: ~1 µg/mL
- Immunoprecipitation: ~1–10 µg/mL
It is recommended to titrate the reagent for optimal performance.
Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
BAX is a member of the Bcl-2 family and plays an important role in regulating apoptosis. While Bcl-2 is anti-apoptotic, BAX promotes apoptosis. Regulation involves homo- and heterodimerization of BAX and Bcl-2 isoforms. The Bax gene encodes isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), both containing BH1, BH2, and BH3 domains. Bax beta has a unique C-terminus lacking a transmembrane domain.
Bcl-2 is also expressed in multiple isoforms; Bcl-2 beta (22 kDa) differs from alpha (26 kDa) in its C-terminus and 3′ UTR.
Overexpression of BAX promotes cell death, and it can form homodimers or heterodimers with other BCL-2-related proteins.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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