Thermo Fisher Scientific Bcl-X Monoclonal Antibody (2H12), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The 2H12 antibody reacts with human, mouse, and rat Bcl-X. Bcl-X functions as a central repressor of several apoptotic signals. This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins.

Applications Reported:
The 2H12 antibody has been reported for use in immunoblotting (WB).

Applications Tested:
Tested by Western blot analysis of HeLa lysate and can be used at less than 2 µg/mL. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities. These proteins are located at the outer mitochondrial membrane and regulate the mitochondrial membrane channel (VDAC) opening. VDAC controls mitochondrial membrane potential, reactive oxygen species production, and cytochrome C release, all of which are key in apoptosis induction. Alternative splicing produces multiple transcript variants encoding two isoforms: the longer isoform acts as an apoptotic inhibitor, and the shorter isoform acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.