Thermo Fisher Scientific Bcl-X Monoclonal Antibody (2H12), eBioscience
인간, 마우스, 랫드 Bcl-X 단백질을 인식하는 단클론 항체로, Bcl-XS와 Bcl-XL 모두 검출 가능. Western blot 및 면역세포염색에 적합하며, 고순도(>90%)와 낮은 응집도(<10%)를 보유. 연구용으로 세포사멸 관련 단백질 분석에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468381 |
Product Specific Information
Description:
The 2H12 antibody reacts with human, mouse, and rat Bcl-X. Bcl-X functions as a central repressor of several apoptotic signals. This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins.
Applications Reported:
The 2H12 antibody has been reported for use in immunoblotting (WB).
Applications Tested:
Tested by Western blot analysis of HeLa lysate and can be used at less than 2 µg/mL. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities. These proteins are located at the outer mitochondrial membrane and regulate the mitochondrial membrane channel (VDAC) opening. VDAC controls mitochondrial membrane potential, reactive oxygen species production, and cytochrome C release, all of which are key in apoptosis induction. Alternative splicing produces multiple transcript variants encoding two isoforms: the longer isoform acts as an apoptotic inhibitor, and the shorter isoform acts as an apoptotic activator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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