Thermo Fisher Scientific V5 Tag Monoclonal Antibody (TCM5), eBioscience
V5 epito프 태그가 포함된 재조합 단백질을 특이적으로 인식하는 TCM5 단클론 항체. Western blot, ICC/IF 등 다양한 응용에 사용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 안정적인 실험 결과 제공. 연구용으로만 사용.
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Thermo Fisher Scientific V5 Tag Monoclonal Antibody (TCM5), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 4 publications |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 5 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TCM5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865243 |
Additional Formats:
Product Specific Information
This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag. The V5 epitope is naturally present on the P and V proteins of the paramyxovirus SV5. TCM5 specifically recognizes the sequence GKPIPNPLLGLDST.
Applications Reported:
- Western blotting
- Immunocytochemistry
Applications Tested:
Tested by western blot and immunofluorescent staining of V5-transfected cells. Recommended usage ≤ 1 µg/mL. Antibody should be titrated for optimal performance.
Purity: Greater than 90%, determined by SDS-PAGE
Aggregation: Less than 10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Epitope tags enable localization of gene products, study of protein topology and complexes, identification of associated proteins, and characterization of low-abundance or poorly immunogenic proteins when protein-specific antibodies are unavailable.
The V5 epitope tag is derived from the P and V proteins of the paramyxovirus SV5. It typically consists of 14 amino acids (GKPIPNPLLGLDST), though shorter variants (9 amino acids, IPNPLLGLD) have also been used.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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