Thermo Fisher Scientific V5 Tag Monoclonal Antibody (TCM5), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

TCM5

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• PE
• PE-Cyanine7
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865243

Product Specific Information

Description: This TCM5 monoclonal antibody recognizes recombinant proteins containing a V5 epitope tag. The V5 epitope is naturally present on the P and V proteins of the paramyxovirus, SV5. TCM5 specifically recognizes the sequence GKPIPNPLLGLDST.

Applications Reported: This TCM5 antibody has been reported for use in western blotting, and immunocytochemistry.

Applications Tested: This TCM5 antibody has been tested by western blot and immunofluorescent staining of V5-transfected cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.