Thermo Fisher Scientific c-Myc Monoclonal Antibody (9E10), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The 9E10 antibody reacts with human c-Myc p67. It was raised against a synthetic peptide corresponding to amino acids 408–439 within the carboxy-terminal domain of human c-Myc. This antibody can detect Myc-tagged proteins.
The transcription factor c-Myc is a proto-oncogene involved in cell cycle regulation, metabolism, apoptosis, differentiation, cell adhesion, and tumorigenesis. In normal cells, c-Myc expression is tightly regulated, but in many human cancers, it is deregulated. c-Myc also plays a key role in apoptosis, particularly in connection with the CD95/Fas death receptor pathway.

Applications Reported:
This 9E10 antibody has been reported for use in immunoprecipitation, western blotting, and immunohistochemistry of formalin-fixed paraffin-embedded tissue sections.

Applications Tested:

• Tested by IHC using low or high pH antigen retrieval at ≤5 µg/mL.
• Tested by western blot at ≤5 µg/mL.
  It is recommended to titrate the antibody for optimal performance in each assay.

Purity: >90%, as determined by SDS-PAGE
Aggregation: <10%, as determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays an important role in proliferation, differentiation, apoptosis, and cell cycle progression.
Phosphorylation at Thr58/Ser62, mediated by kinases such as GSK3, CDK, ERK2, and JNK, regulates c-Myc function. c-Myc is essential for vasculogenesis and angiogenesis in tumor development.
The c-myc oncogene is involved in normal cellular proliferation and differentiation, while deregulated expression induces apoptosis. Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags.
Amplification of c-Myc occurs in several human tumors, including lung, breast, and colon carcinomas. Translocation of the c-myc locus on chromosome 8 to immunoglobulin loci is characteristic of Burkitt’s lymphoma and other B-cell conditions. Aberrant expression is found in tumors of multiple origins, including colorectal, gastric, hepatic, ovarian, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.