Thermo Fisher Scientific USP7 Monoclonal Antibody (USP 3D8), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/test

-

Immunocytochemistry (ICC/IF)

1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

USP 3D8

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2784638

Product Specific Information

Description: This USP 3D8 monoclonal antibody recognizes human USP7, an ubiquitin specific protease. USP 3D8 detects a ~128 kDa band corresponding to USP7 by Western Blot and stains USP7 in the nucleus in Immunocytochemistry/immunofluorescence.

Applications Reported: This USP 3D8 antibody has been reported for use in western blot and immunocytochemistry/immunofluorescence.

Applications Tested: This USP 3D8 antibody has been tested by western blot of human cell lysates and immunocytochemistry/immunofluorescence of human cell lines. This may be used at less than or equal to 2 µg/mL for western blot and less than or equal to 2 µg/mL for immunocytochemistry/immunofluorescence.

Target Information

USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low in part due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.