Thermo Fisher Scientific DNMT3B Monoclonal Antibody (7E5E9C1)
DNMT3B 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, Flow cytometry, ELISA 등에 적합. Human 시료에 반응하며, Protein G로 정제된 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E5E9C1 |
| Immunogen | Purified recombinant fragment of human DNMT3B (aa 1–150) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898425 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
CpG methylation is an epigenetic modification important for embryonic development, imprinting, and X-chromosome inactivation. DNA methylation is essential for mammalian development. The DNMT3B gene encodes a DNA methyltransferase functioning in de novo methylation. The protein localizes mainly to the nucleus and shows developmentally regulated expression. Mutations in this gene cause immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described, though full-length sequences of variants 4 and 5 remain undetermined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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