Thermo Fisher Scientific Phospho-WNK1 (Thr60) Polyclonal Antibody
WNK1 단백질의 Thr60 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며, 인간, 생쥐, 랫트 시료에 반응. 혈압 및 전해질 항상성 연구에 활용 가능.
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Thermo Fisher Scientific Phospho-WNK1 (Thr60) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human WNK1 (Accession Q9H4A3), corresponding to amino acid residues around phosphorylated Thr60 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816506 |
Product Specific Information
This antibody detects endogenous levels of WNK1 only when phosphorylated at Threonine 60.
Target Information
WNK1 serine/threonine kinase is a member of the WNK subfamily and may regulate blood pressure by controlling sodium and chloride ion transport. Mutations in this gene are associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II.
WNK1 plays key roles in electrolyte homeostasis, cell signaling, survival, and proliferation. It acts as both activator and inhibitor of sodium- and potassium-coupled chloride cotransporters, respectively, and activates SCNN1A, SCNN1B, SCNN1D, and SGK1.
WNK1 localizes to the distal nephron, a kidney segment involved in salt, potassium, and pH balance. Mutations in WNK genes can result in pseudohypoaldosteronism and neuropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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