Thermo Fisher Scientific Phospho-DARPP-32 (Thr75) Polyclonal Antibody
DARPP-32 Thr75 인산화 상태를 특이적으로 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 정제 및 -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human PPP1R1B (Accession Q9UD71), corresponding to amino acid residues around phosphorylated Thr75 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816510 |
Product Specific Information
This antibody detects endogenous levels of DARPP-32 only when phosphorylated at Threonine 75.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regulated ~32 kDa phosphoprotein associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when phosphorylated on Thr34, and acts as an inhibitor of PKA when phosphorylated on Thr75. Phosphorylation of DARPP-32 plays a critical role in dopaminergic neurotransmission and is also implicated in the actions of alcohol, caffeine, and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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