Thermo Fisher Scientific Phospho-WNK1 (Thr60) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human WNK1(Accession Q9H4A3), corresponding to amino acid residues around phosphorylated Thr60. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WNK1,uniProtId:Q9H4A3-1,ncbiNodeId:9606,antigenRange:60,antigenLength:2382,antigenImageFileName:PA5-105033_WNK1_Q9H4A3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-105033_WNK1_Q9H4A3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816506

Product Specific Information

Antibody detects endogenous levels of WNK1 only when phosphorylated at Threonine 60.

Target Information

WNK1 serine/threonine kinase is a member of the WNK subfamily. WNK1 may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Also, acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, and SGK1. WNK localizes to a segment of kidney involved in salt, potassium, and pH homeostasis, the distal nephron. Mutations in the WNK gene result in Pseudohypaldosteronism and Neuropathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.