Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 650
FOXP3 단백질을 인식하는 DyLight 650 결합 토끼 폴리클로날 항체. Western blot 및 면역조직화학(IHC) 분석에 적합. 인간 시료에서 약한 반응성을 보임. 4°C 어둠 속 보관, 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to the N-terminal region of human FOXP3 (between residues 1–50). |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 3.76 mg/mL |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154472 |
Product Specific Information
This antibody reacts weakly with human samples.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis.
FOXP3 is 381 amino acids long, expressed at high levels in CD25⁺ CD4⁺ regulatory T cells, low in CD4⁺/CD25⁻ cells, and absent in CD4⁻/CD8⁺ T cells.
It functions as a master regulatory gene and a specific marker of regulatory T cells.
Defects in FOXP3 cause the scurfy phenotype in mice and are associated with IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome) in humans.
In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
Transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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