Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 488
FOXP3 단백질을 표적하는 DyLight 488 결합 폴리클로날 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 488
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide from C-terminal region of human FOXP3 (residues 400–431) |
| Conjugate | DyLight™ 488 |
| Excitation/Emission Max | 492 / 519 nm |
| Form | Liquid |
| Concentration | 0.74 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11157267 |
Product Specific Information
This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is expressed at high levels in CD25⁺ CD4⁺ regulatory T cells, low levels in CD4⁺/CD25⁻ cells, and absent in CD4⁻/CD8⁺ T cells. FOXP3 may act as a master regulatory gene and a specific marker of regulatory T cells.
Mutations in FOXP3 cause the scurfy phenotype in mice and are associated with IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. Transcript variants encoding different isoforms have been identified. In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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