Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 550
FOXP3 단백질을 인식하는 DyLight 550 결합 폴리클로날 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능하며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400–431) |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.69 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156415 |
Product Specific Information
This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25+ CD4+ regulatory T cells, at a low level in CD4+/CD25– cells, and is absent in CD4–/CD8+ T cells. FOXP3 functions as a master regulatory gene and a specific marker of regulatory T cells. Defects in FOXP3 cause the scurfy phenotype in mice and are implicated in human IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. In human breast and colon cancer cells, FOXP3 expression is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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