Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 650
FOXP3 단백질을 검출하기 위한 DyLight 650 결합 폴리클로날 항체로, 인간 및 마우스 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific FOXP3 Polyclonal Antibody, DyLight 650
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide made to the C-terminal region of human FOXP3 (between residues 400–431) |
| Conjugate | DyLight™ 650 |
| Excitation/Emission Max | 651/673 nm |
| Form | Liquid |
| Concentration | 0.85 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154329 |
Product Specific Information
This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator belonging to the forkhead/winged-helix family, essential for immune homeostasis and conserved across mammals. It is expressed at high levels in CD25⁺ CD4⁺ regulatory T cells, low in CD4⁺/CD25⁻ cells, and absent in CD4⁻/CD8⁺ T cells. FOXP3 functions as a master regulatory gene and a specific marker of regulatory T cells. Mutations in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome) in humans. Expression of FOXP3 in human breast and colon cancer cells is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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