Thermo Fisher Scientific Progerin Monoclonal Antibody (13A4D4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200- 1:500

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

13A4D4

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 604-611 (GAQSPQNC) at the C terminus of Human Progerin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Progerin,uniProtId:P02545-1,ncbiNodeId:9606,antigenRange:604-611,antigenLength:664,antigenImageFileName:MA5-18073_Progerin_P02545-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18073_Progerin_P02545-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539456

Product Specific Information

This antibody specifically recognizes Progerin (Lamin A delta50) and does not cross-react with endogenous prelamin A.

Western blot results show a band at approximately 70 kDa.

Target Information

Progerin is a 614 amino acid protein involved in Hutchinson-Gilford progeria syndrome. Progerin is most often generated by a point mutation (C1824T) in the LMNA gene that codes for lamin-A/C. This mutation activates a cryptic splice site and gives rise to a form of lamin A with a 50-amino acid internal deletion within the C-terminal domain of the protein. Approximately 80% of Hutchinson-Gilford progeria syndrome cases carry a single copy of the most common mutation, a silent point mutation, G608G (GGC > GGT), within exon 11 of the LMNA gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.