Thermo Fisher Scientific Progerin Monoclonal Antibody (13A4D4)
Progerin 단백질을 특이적으로 인식하는 mouse monoclonal 항체로, 인간 시료에 반응합니다. Western blot, ICC/IF, ELISA, IP에 사용 가능하며, 비결합형 액상 형태로 제공됩니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:200–1:500 | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13A4D4 |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 604–611 (GAQSPQNC) at the C-terminus of Human Progerin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539456 |
Product Specific Information
This antibody specifically recognizes Progerin (Lamin A delta50) and does not cross-react with endogenous prelamin A.
Western blot results show a band at approximately 70 kDa.
Target Information
Progerin is a 614 amino acid protein involved in Hutchinson-Gilford progeria syndrome. It is generated by a point mutation (C1824T) in the LMNA gene coding for lamin-A/C. This mutation activates a cryptic splice site, producing a lamin A form with a 50-amino acid internal deletion within the C-terminal domain. Approximately 80% of Hutchinson-Gilford progeria syndrome cases carry a single copy of the most common silent point mutation, G608G (GGC > GGT), within exon 11 of the LMNA gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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