Thermo Fisher Scientific Nesprin 1 Monoclonal Antibody (MANNES1A(7A12))

Applications

Tested Dilution

Publications

Western Blot (WB)

1:400-1:800

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:200

View 7 publications 7 publications

Immunomicroscopy (IM)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MANNES1A(7A12)

Immunogen

Recombinant full length Nesprin 1(Human) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nesprin 1,uniProtId:Q8NF91-1,ncbiNodeId:9606,antigenRange:1-8797,antigenLength:8797,antigenImageFileName:MA5-18077_Nesprin_1_Q8NF91-1_House_mouse.svg,antigenImageFileNamePDP:MA5-18077_Nesprin_1_Q8NF91-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539460

Product Specific Information

This antibody recognizes the C-terminal region of nesprin-1.

This target has a predicted molecular weight of 1011 kDa.

Target Information

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called nesprin-l and -2 for nuclear _envelope _spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin-l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as dystrophins of the nucleus to maintain nuclear organization and structura integrity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.