Thermo Fisher Scientific Nesprin 1 Monoclonal Antibody (MANNES1A(7A12))

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody recognizes the C-terminal region of nesprin-1.
The target has a predicted molecular weight of approximately 1011 kDa.

Target Information

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus.

These proteins are called nesprin-1 and -2 (nuclear envelope spectrin repeat), characterized by multiple clustered spectrin repeats, bipartite nuclear localization sequences, and a conserved C-terminal single transmembrane domain.

Transient transfection of EGFP-fusion constructs demonstrated localization to the nuclear membrane with a novel C-terminal TM-domain-containing sequence essential for perinuclear localization. Nesprin-1 is developmentally regulated in smooth and skeletal muscle and relocates from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation.

Nesprins may function as “dystrophins of the nucleus” to maintain nuclear organization and structural integrity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.