Thermo Fisher Scientific FGF23 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• 사용 농도: 0.1–0.2 µg/mL

ELISA

• 사용 농도: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-23. Anti-Human FGF-23-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human FGF-23 by sandwich ELISA (using 100 µL/well), use 0.25–1.0 µg/mL of this antibody.
When used with PeproTech Polyclonal Anti-Human FGF-23 (500-P319) as a capture antibody, detection sensitivity is 2000–4000 pg/mL of Recombinant Human FGF-23.

Western Blot:
To detect Human FGF-23 by Western Blot, use 0.1–0.2 µg/mL.
Detection limit: 1.5–3.0 ng/lane (reducing or non-reducing conditions).

Package Information:
500-P319BT-1MG is provided as 2 × 500 µg.

Target Information

The FGF23 gene encodes a member of the fibroblast growth factor family, which has mitogenic and cell survival activities.
FGF family members are involved in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion.
FGF23 inhibits renal tubular phosphate transport and is associated with autosomal dominant hypophosphatemic rickets and oncogenic hypophosphatemic osteomalacia.
Mutations in this gene can also cause familial tumoral calcinosis with hyperphosphatemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.