Thermo Fisher Scientific TNF alpha Polyclonal Antibody, Biotin, PeproTech
인간 TNF-alpha를 검출하기 위한 비오틴 결합 폴리클로날 항체로, Western blot과 ELISA에 최적화되어 있습니다. 고순도 항원으로 면역화된 토끼 혈청에서 생산되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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- 500-P31ABT-xxxx (2개 옵션)
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Thermo Fisher Scientific TNF alpha Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | 3 publications |
| Neutralization (Neu) | - | 1 publication |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 17.4 kDa Recombinant Human TNF-alpha |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930375 |
Product Specific Information
AA Sequence of recombinant protein:
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TNF-alpha. Anti-Human TNF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human TNF-alpha by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TNF-alpha (500-P31A) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human TNF-alpha.
Western Blot:
To detect Human TNF-alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human TNF-alpha is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P31ABT-1MG will be provided as 2 × 500 µg.
Target Information
TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.
It exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice suggest a neuroprotective function. TNF alpha induces tumor necrosis in tumor-bearing mice and plays roles in immune responses to bacterial, viral, parasitic, and fungal infections, as well as tumor cytolysis, synergistic with interferon-gamma.
This cytokine has been implicated in autoimmune diseases, insulin resistance, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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