Thermo Fisher Scientific TNF alpha Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific TNF alpha Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TNF-alpha. Anti-Human TNF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human TNF-alpha by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TNF-alpha (500-P31A) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human TNF-alpha.

Western Blot:
To detect Human TNF-alpha by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human TNF-alpha is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P31ABT-1MG will be provided as 2 × 500 µg.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine belonging to the tumor necrosis factor (TNF) superfamily. It is mainly secreted by macrophages and binds to receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha regulates immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

It exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice suggest a neuroprotective function. TNF alpha induces tumor necrosis in tumor-bearing mice and plays roles in immune responses to bacterial, viral, parasitic, and fungal infections, as well as tumor cytolysis, synergistic with interferon-gamma.

This cytokine has been implicated in autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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