Thermo Fisher Scientific FGF23 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific FGF23 Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-23. Anti-Human FGF-23-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human FGF-23 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-23 (500-P319) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human FGF-23.

Western Blot:
To detect Human FGF-23 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human FGF-23 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P319BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in various biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion.
FGF23 inhibits renal tubular phosphate transport. Mutations in this gene are associated with autosomal dominant hypophosphatemic rickets and familial tumoral calcinosis. Abnormally high expression levels have been found in oncogenic hypophosphatemic osteomalacia.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.