Thermo Fisher Scientific TFAM Polyclonal Antibody
TFAM 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, IP 등에 사용 가능. Human, Mouse, Rat 반응성. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 버퍼에 보존. 미토콘드리아 DNA 복제 및 전사 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length human mtTFA recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.43 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547047 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro 2A
- Predicted reactivity: Chimpanzee (99%)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial transcription factor A (TFAM) is a crucial protein encoded by nuclear genes but transported from the cytoplasm to mitochondria, where it plays a key role in the maintenance and expression of mitochondrial DNA (mtDNA). It regulates replication and transcription of mtDNA, ensuring proper mitochondrial function.
TFAM contains an LC3 interacting region (LIR) motif that binds autophagy proteins such as LC3, essential for the autolysosomal pathway to eliminate leaked mtDNA and limit inflammation. TFAM levels vary across tissues, influencing mitochondrial function. High TFAM levels may repress mtDNA expression in skeletal muscle, leading to OXPHOS deficiencies, while in the heart, increased mtDNA copy number maintains balance and preserves OXPHOS capacity.
Disruptions in TFAM are associated with neurodegeneration and diseases linked to mtDNA stress.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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