Thermo Fisher Scientific UCP1 Polyclonal Antibody
Thermo Fisher Scientific의 UCP1 Polyclonal Antibody는 인간 및 생쥐에서 반응하는 항체로 Western blot 및 IHC에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 형태이며, 미토콘드리아 탈결합 단백질 연구에 사용됩니다. 연구용 전용 제품입니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | View publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 1–299 of Human UCP1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.34 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2547051 |
Product Specific Information
- Recommended positive control: mouse brown adipose tissue
- Predicted reactivity: Mouse (80%), Rat (80%), Rabbit (86%), Sheep (83%), Bovine (84%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial uncoupling proteins (UCPs) are members of the mitochondrial anion carrier protein family. They separate oxidative phosphorylation from ATP synthesis, dissipating energy as heat through the mitochondrial proton leak. UCPs facilitate anion transfer from the inner to the outer mitochondrial membrane and proton return transfer, reducing mitochondrial membrane potential in mammalian cells.
UCPs contain three homologous protein domains of MACPs. The UCP1 gene is expressed specifically in brown adipose tissue, which functions to produce heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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