Thermo Fisher Scientific Lamin A Monoclonal Antibody (133A2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

1:100-1:200

-

Product Specifications

Species Reactivity

Bovine, Dog, Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

133A2

Immunogen

Partially purified recombinant human lamin A. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Lamin A,uniProtId:P02545-1,ncbiNodeId:9606,antigenRange:1-664,antigenLength:664,antigenImageFileName:MA1-06101_Lamin_A_P02545-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06101_Lamin_A_P02545-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_559889

Product Specific Information

MA1-06101 detects lamin A in human, mouse, rat, bovine and canine samples.

MA1-06101 has sucessfully been used in immunocytochemistry, immunohistochemistry (paraffin), flow cytometry, and Western blotting procedures.

The MA1-06101 immunogen is partially purified recombinant human lamin A.

Target Information

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.