Thermo Fisher Scientific Snail1 Monoclonal Antibody (20C8), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific Snail1 Monoclonal Antibody (20C8), Alexa Fluor 488, eBioscience™

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug belong to a family of transcription factors responsible for inducing epithelial-to-mesenchymal transition (EMT) during organ development and tumor progression.
Snail1 is localized in the cytoplasm and nucleus, functioning as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is expressed in the mesoderm; in adults, expression is inducible and typically limited to activated fibroblasts and some mesenchymal cells. Overexpression has been observed in carcinomas and fibrosarcomas, serving as an early EMT marker.

Applications Reported:
Immunohistochemistry (IHC-P), ELISA, immunoprecipitation, western blotting, immunocytochemistry, and flow cytometric intracellular staining.

Applications Tested:
Tested by ICC on fixed/permeabilized MCF7 cells and IHC on formalin-fixed paraffin-embedded human infiltrating ductal carcinoma. Recommended ≤20 µg/mL, with titration advised for optimal performance.

Fluorescence Properties:
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Zinc finger protein SNAI1 (SNAIL) is a transcriptional repressor that downregulates ectodermal gene expression within the mesoderm, contributing to mesoderm and neural crest formation.
SNAI1 plays a key role in tumor invasion through E-cadherin downregulation and EMT induction. In breast cancer, SNAI1 and SNAI2 (Slug) expression correlates with E-cadherin repression, metastasis, recurrence, and poor prognosis. Snail1 protein in stroma may serve as a potential prognostic marker for colon tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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