Thermo Fisher Scientific Snail1 Monoclonal Antibody (20C8), Alexa Fluor 488, eBioscience
인간 Snail1 단백질을 인식하는 Alexa Fluor 488 결합 단일클론 항체입니다. EMT 연구, 암 전이 및 E-cadherin 발현 억제 기전 연구에 적합합니다. IHC, ICC, WB, ELISA 등 다양한 응용에 사용 가능합니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific Snail1 Monoclonal Antibody (20C8), Alexa Fluor 488, eBioscience™
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | 6 publications |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 20 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 20 µg/mL | 4 publications |
| Flow Cytometry (Flow) | Assay-Dependent | - |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20C8 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574483 |
Product Specific Information
The monoclonal antibody 20C8 recognizes human Snail1, a 29 kDa zinc finger transcription factor. Snail1 and Slug belong to a family of transcription factors responsible for inducing epithelial-to-mesenchymal transition (EMT) during organ development and tumor progression.
Snail1 is localized in the cytoplasm and nucleus, functioning as a transcriptional repressor of E-cadherin, claudins, occludin, and desmoplakin. During embryonic development, Snail1 is expressed in the mesoderm; in adults, expression is inducible and typically limited to activated fibroblasts and some mesenchymal cells. Overexpression has been observed in carcinomas and fibrosarcomas, serving as an early EMT marker.
Applications Reported:
Immunohistochemistry (IHC-P), ELISA, immunoprecipitation, western blotting, immunocytochemistry, and flow cytometric intracellular staining.
Applications Tested:
Tested by ICC on fixed/permeabilized MCF7 cells and IHC on formalin-fixed paraffin-embedded human infiltrating ductal carcinoma. Recommended ≤20 µg/mL, with titration advised for optimal performance.
Fluorescence Properties:
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Zinc finger protein SNAI1 (SNAIL) is a transcriptional repressor that downregulates ectodermal gene expression within the mesoderm, contributing to mesoderm and neural crest formation.
SNAI1 plays a key role in tumor invasion through E-cadherin downregulation and EMT induction. In breast cancer, SNAI1 and SNAI2 (Slug) expression correlates with E-cadherin repression, metastasis, recurrence, and poor prognosis. Snail1 protein in stroma may serve as a potential prognostic marker for colon tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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