Thermo Fisher Scientific IRF8 Monoclonal Antibody (V3GYWCH), Alexa Fluor 488, eBioscience

Applications

• Flow Cytometry (Flow): Tested at ≤ 0.25 µg/test
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Product Specifications

Additional Formats

• APC
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor essential for the development of dendritic cells (DC), particularly plasmacytoid DC (pDC) and CD8α⁺ DC. IRF8⁻/⁻ mice lack both pDC and CD8α⁺ DC populations. Although CD8α⁻ DC are present, they fail to mature upon Toll-like receptor signaling and are functionally impaired. Retroviral IRF8 transduction can restore pDC development from DC progenitors in IRF8⁻/⁻ mice.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤ 0.25 µg/test (100 µL final volume). Cell number should be optimized empirically (10⁵–10⁸ cells/test).
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser

Target Information

IRF8 (interferon regulatory factor 8) functions as a transcriptional activator or repressor, binding to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. It plays a key negative regulatory role in immune cells and is involved in CD8⁺ dendritic cell differentiation through complex formation with the BATF-JUNB heterodimer. This interaction recognizes the AICE sequence, leading to cooperative gene activation. Mutations in IRF8 can cause immunodeficiency 32A and 32B.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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