Thermo Fisher Scientific c-MAF Monoclonal Antibody (sym0F1), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• View 2 publications

Product Specifications

Available Conjugate Formats

• Brilliant Violet 421
• Brilliant Violet 480
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The sym0F1 monoclonal antibody reacts with human and mouse c-Maf, a 42 kDa basic leucine zipper transcription factor involved in neural, ocular, and hematopoietic systems. In hematopoietic cells, c-Maf is crucial for IL-4 expression in Th2 cells and plays roles in Th17 and Tfh cell differentiation. It drives IL-21 expression and regulates IL-23R (Th17) and CXCR5 (Tfh) expression.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
• Recommended Protocol: Staining Intracellular Antigens for Flow Cytometry, Protocol B
• Usage: ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Excitation: 488 nm
• Emission: 519 nm
• Laser: Blue Laser

Target Information

c-Maf is the cellular counterpart of oncogenic v-Maf, a basic region leucine zipper transcription factor. It interacts with LRPICD and exists as two mRNA forms (long and short). c-Maf regulates IL-4, crystalline, insulin, p53, and L7 gene expression through Maf recognition elements (MARE). It is involved in Th2 differentiation, hematopoiesis, and IL-10 gene expression in macrophages. Chromosomal aberrations involving maf are associated with multiple myeloma, and it is expressed in myeloma cell lines and resting monocytes/macrophages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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