
Takara iDimerize Reverse Dimerization System (inducible secretion)
✨AI 추천 연관 상품
AI가 분석한 이 상품과 연관된 추천 상품들을 확인해보세요
연관 상품을 찾고 있습니다...
The iDimerize Reverse Dimerization System brings the disruption of protein complexes under real-time, small-molecule control. A protein of interest is fused to the DmrD binding domain, and the fusion protein molecules aggregate unless the D/D Solubilizer ligand is present. Plasmid and lentiviral (Lenti-X) vector formats are available.
The iDimerize Reverse Dimerization System brings the disruption of protein complexes under real-time, small-molecule control. A protein of interest is fused to the DmrD binding domain, and the fusion protein molecules aggregate unless the D/D Solubilizer ligand is present. Plasmid and lentiviral (Lenti-X) vector formats are available.
Reverse dimerization: Disrupting protein-protein interactions
The iDimerize Reverse Dimerization System is a “reverse dimerization” system—aggregation is the resting state, and the D/D Solubilizer breaks up protein-protein interactions. Therefore, the iDimerize Reverse Dimerization System complements inducible dimerization, and can be used in analogous ways to create inducible alleles. In principle, most processes that can be brought under dimerizer control can also be controlled in the reverse manner using this kit to turn off a process that is activated by oligomerization.
Inducible secretion
The ability to create large protein aggregates has unique applications. For example, adding a secretory signal sequence to fusion proteins allows them to be reversibly stored as aggregates in the endoplasmic reticulum. The ligand can then be added to induce a rapid pulse of protein secretion from the cells. This method has been used to induce rapid, transient, and tightly regulated secretion of human growth hormone (hGH) and insulin (Rivera et al. 2000).
Protein aggregates can also be used in protein trafficking research. For example, this approach has been used to discover the existence of “mega-vesicles” transporting cargo across the Golgi stack (Volchuk et al. 2000).
D/D Solubilizer ligand
The D/D Solubilizer is a synthetic, cell-permeable ligand that can be used to disrupt dimerization of fusion proteins containing the DmrD domain. The D/D Solubilizer has been tested in vitro and in mice. It is nontoxic. We suggest testing various D/D Solubilizer concentrations within the recommended range (10–500 nM) for different lengths of time (30 minutes to 12+ hours) in order to obtain a complete dose-response profile.
🏷️Takara 상품 둘러보기
동일 브랜드의 다른 상품들을 확인해보세요

Takara
Takara MicroRNA first-strand synthesis and miRNA quantitation kits
455,000원

Takara
Takara Soluble protein expression: single-protein production system
1,258,000원

Takara
Takara iDimerize Reverse Dimerization System (inducible secretion)
571,000원

Takara
Takara Flow cytometer calibration beads for AcGFP1/EGFP and mCherry
663,000원

Takara
Takara Diversify PCR Random Mutagenesis Kit for sequence mutations
1,580,000원
배송/결제/교환/반품 안내
배송 정보
기본 배송비 |
| 교환/반품 배송비 |
|
---|---|---|---|
착불 배송비 |
| ||
교환/반품 배송비 |
|
결제 및 환불 안내
결제수단 |
|
---|---|
취소 |
|
반품 |
|
환급 |
|
교환 및 반품 접수
교환 및 반품 접수 기한 |
|
---|---|
교환 및 반품 접수가 가능한 경우 |
|
교환 및 반품 접수가 불가능한 경우 |
|
교환 및 반품 신청
교환 절차 |
|
---|---|
반품 절차 |
|