Takara MicroRNA first-strand synthesis and miRNA quantitation kits

Mir-X miRNA qRT-PCR TB Green Kits are complete systems for microRNA quantitation by qPCR from total RNA or purified small RNA samples. Each kit is complete and includes reagents for both:

• First-strand cDNA synthesis from miRNA
• Quantitative PCR (qPCR) of miRNA

Mir-X miRNA qRT-PCR TB Green Kits are complete systems for microRNA quantitation by qPCR from total RNA or purified small RNA samples. Each kit is complete and includes reagents for both:

• First-strand cDNA synthesis from miRNA and
• Quantitative PCR (qPCR) of miRNA

Simple, single-step cDNA synthesis

Unlike other vendors` kits, these microRNA quantitation kits provide a simple, single-step protocol for the first-strand synthesis reaction. Mir-X uses an optimized mix of poly(A) polymerase and SMART MMLV Reverse Transcriptase to synthesize first-strand cDNA from your microRNA-containing RNA sample.

Sensitive qPCR using microRNA-specific primers

cDNA is amplified and quantified by qPCR using your microRNA-specific primer and our TB Green Advantage qPCR Premix. Multiple microRNA species, as well as the mRNA targets of the microRNAs, can be amplified from a single cDNA sample. The system is able to detect microRNAs down to 50 copies.

Highly specific detection of microRNA

To demonstrate the specificity of Mir-X miRNA quantitation, we used a series of 8 highly similar synthetic Let7 miRNA variants. We first spiked each of the Let7 miRNAs into separate samples of yeast polyA+ RNA and generated cDNA using the Mir-X single-tube reaction. We then tested a panel of variant-specific primers with each cDNA sample to determine each primer’s ability to specifically and individually quantify the Let7 subtypes in the cDNA sample. Despite the high degrees of similarity among the variants and the primers, Mir-X qPCR was highly specific in detecting each Let7 variant.

Large format qPCR kits

The kits are available in economical, large-sized formats that provide 200 or 600 qPCR reactions, and each kit includes our Mir-X miRNA First-Strand Synthesis Kit and TB Green Advantage qPCR Premix.

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Overview

• Simple, single-step cDNA synthesis reaction
• Two kits in one: cDNA synthesis and qPCR of microRNA
• Quantify any microRNA and its target using the same RNA sample
• Detection of microRNA down to 50 copies
• High specificity
• 200 rxn and 600 rxn qPCR kit formats

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Applications

• cDNA synthesis from microRNA
• microRNA quantitation by qPCR

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.