Takara Diversify PCR Random Mutagenesis Kit for sequence mutations

Takara Diversify PCR Random Mutagenesis Kit

제품 개요

The Diversify PCR Random Mutagenesis Kit employs a nontoxic method for mutagenizing sequences of up to 4.0 kb in length. The kit broadly distributes all possible mutations without creating hot spots. By allowing you to manipulate mutagenic conditions, the Diversify PCR Random Mutagenesis Kit provides optimal mutagenesis of sequences over an exceptionally broad size range.
Select mutation rates from two to eight mutations per 1,000 bp simply by varying the amounts of two key reagents—manganese (Mn2+) and dGTP (Cadwell and Joyce 1992; Leung, Chen, and Goeddel 1989).

The kit contains the Titanium Taq PCR system to reliably amplify DNA fragments of up to 4.0 kb in length. This extended amplification range makes the Diversify kit ideal for mutating operons, plasmids, and sequences corresponding to large proteins, in addition to short DNA fragments.
It also includes a rapid control reaction that allows relative comparison of mutation rates in the control fragment within two hours following PCR. In addition to the Titanium Taq polymerase mix, the kit provides all reagents and buffers needed for mutagenesis.

주요 특징

• Controlled, random mutagenesis for investigating protein function
• Precise control of mutation rate
• No mutational hot spots
• Obtain all possible base substitutions

응용 분야

• Random mutagenesis

참고 문헌

Cadwell, R. C. & Joyce, G. F. Randomization of genes by PCR mutagenesis. PCR Methods Appl. 2, 28–33 (1992).
Leung, D., Chen, E., and Goeddel, D. V. PCR-based random mutagenesis using skewed nucleotide concentrations. Technique 1, 1–15 (1989).

제품 인용

Mo, J. Y., Maki, H. & Sekiguchi, M. Mutational specificity of the dnaE173 mutator associated with a defect in the catalytic subunit of DNA polymerase III of Escherichia coli. J. Mol. Biol. 222, 925–36 (1991).

추가 정보

Please see the product’s Certificate of Analysis for information about storage conditions, product components, and technical specifications.
Refer to the Kit Components List under the Documents tab for detailed component information.

제품 사양