Thermo Fisher Scientific HSPB8 Monoclonal Antibody (3C12-H11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3C12-H11

Immunogen

Full-length his-tagged human recombinant HSP22 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSPB8,uniProtId:Q9UJY1-1,ncbiNodeId:9606,antigenRange:1-196,antigenLength:196,antigenImageFileName:MA5-27706_HSPB8_Q9UJY1-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27706_HSPB8_Q9UJY1-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735223

Product Specific Information

1 µg/mL of MA5-27706 was sufficient for detection of HSP22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 22kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.

Target Information

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.