Thermo Fisher Scientific CENPA Monoclonal Antibody (5A7-2E11)
CENPA 단백질을 검출하기 위한 Mouse IgG1 단클론 항체. Western blot 및 Immunocytochemistry에 사용 가능. 고순도 Protein G 정제, 액상 형태로 제공. 인간 시료 반응성, 안정적인 -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A7-2E11 |
| Immunogen | Synthetic peptide corresponding to a portion of human CENP-A (NH2-PRRRSRKPEAPRRRSPS-COOH) (aa 3-19) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735143 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-27710 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 18 kDa. Recognizes Human CENP-A.
Target Information
Centromeres are differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein containing a histone H3-related histone fold domain required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces one or both copies of conventional histone H3 in the (H3-H4)₂ tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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