Thermo Fisher Scientific CaV1.2 Monoclonal Antibody (S57)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

1:200

-

Antibody Microarray (AM)

Assay-dependent

-

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rabbit, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

S57

Immunogen

Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1.2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CaV1.2,uniProtId:P15381-1,ncbiNodeId:9986,antigenRange:1507-1733,antigenLength:2171,antigenImageFileName:MA5-27717_CaV1.2_P15381-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27717_CaV1.2_P15381-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• FITC
• PE
• PerCP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735138

Product Specific Information

1 µg/mL of MA5-27717 was sufficient for detection of Cav1.2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 240kDa (varies with cell background due to glycosylation).

Target Information

CACNA1C encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by CACNA1C binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.