Thermo Fisher Scientific ATP7B Monoclonal Antibody (L62/29)
ATP7B 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 단백질 G 정제, 1 mg/mL 농도, -20°C 보관. Wilson 병 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | L62/29 |
| Immunogen | Synthetic peptide amino acids 3–21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735126 |
Additional Formats
- APC: MA5-45522
- FITC: MA5-45523
- PE: MA5-45525
- PerCP: MA5-45524
- Custom conjugation available upon request
Product Specific Information
- 1 µg/mL of MA5-27719 was sufficient for detection of Copper-transporting ATPase2 in 20 µg of rat brain lysate by colorimetric immunoblot using Goat IgG:HRP as the secondary antibody.
- Detects approximately 160 kDa in rat brain membrane preparations.
- This antibody was formerly sold as clone S62-29.
Target Information
ATP7B is a member of the P-type cation transport ATPase family. It encodes a membrane protein with multiple transmembrane domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least two copper-binding sites. The protein functions as a monomer responsible for exporting copper from cells, including hepatic copper efflux into bile.
Alternative splicing produces different isoforms with distinct cellular localizations. Mutations in ATP7B are associated with Wilson disease (WD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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