Thermo Fisher Scientific GLI3 Recombinant Rabbit Monoclonal Antibody (8B1E2)
GLI3 단백질을 표적하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, ELISA에 적합합니다. HEK293 발현 시스템 기반으로 높은 특이성과 재현성을 제공합니다. 인체, 마우스, 랫트 반응성이 확인되었으며 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8B1E2 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1301–1580 of human Gli3 (P10071) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911942 |
Product Specific Information
Positive test controls include: HeLa.
Immunogen sequence:
PNESAGSMVN GMQNQDPVGQ GYLAHQLLGD SMQHPGAGRP GQQMLGQISA TSHINIYQGP ESCLPGAHGM GSQPSSLAVV RGYQPCASFG GSRRQAMPRD SLALQSGQLS DTSQTCRVNG IKMEMKGQPH PLCSNLQNYS GQFYDQTVGF SQQDTKAGSF SISDASCLLQ GTSAKNSELL SPGANQVTST VDSLDSHDLE GVQIDFDAII DDGDHSSLMS GALSPSIIQN LSHSSSRLTT PRASLPFPAL SMSTTNMAIG DMSSLLTSLA EESKFLAVMQ
Target Information
This gene encodes a protein belonging to the C2H2-type zinc finger subclass of the Gli family.
They function as DNA-binding transcription factors and mediate Sonic hedgehog (Shh) signaling.
The encoded protein localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression.
It is also implicated in embryogenesis.
Mutations in this gene are associated with Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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