Thermo Fisher Scientific PRAS40 Recombinant Rabbit Monoclonal Antibody (2Y8Y3)
PRAS40 단백질을 인식하는 토끼 리콤비넌트 단일클론 항체로, Western blot 및 ELISA에 적합합니다. 인간 및 생쥐 시료에 반응하며, HEK293 세포에서 발현되었습니다. Affinity Chromatography로 정제되어 높은 특이성과 재현성을 제공합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 2Y8Y3 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 150–256 of human AKT1S1/PRAS40 (Q96B36) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.05 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911947 |
Product Specific Information
- Positive test controls: HeLa, MCF7, NIH/3T3, RD
- Localization: Cytoplasm, cytosol
Immunogen sequence:
STDDGSLSEE TPAGPPTCSV PPASALPTQQ YAKSLPVSVP VWGFKEKRTE ARSSDEENGP PSSPDLDRIA ASMRALVLRE AEDTQVFGDL PRPRLNTSDF QKLKRKY
Target Information
The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1 (PRAS40) is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia.
AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy.
Treatment with insulin-like growth factor-1 (IGF1) can induce phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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