Thermo Fisher Scientific Phospho-Elk1 (Ser389) Polyclonal Antibody
Phospho-Elk1 (Ser389) 항체로, 인간·마우스·랫트 시료에서 ELK1 인산화 상태 검출에 적합. WB 및 IHC에 사용 가능하며, Protein A로 정제된 1 mg/mL 액상 형태. -20°C 보관, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence including phosphorylation site of Serine 389 (P-R-S(p)-P-A) derived from human Elk1 and conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | Dulbecco’s PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. It is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation, particularly at Ser383, is critical for transcriptional activation by ELK1. ELK1 serves as a direct target of activated MAP kinase and acts as a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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