Thermo Fisher Scientific Phospho-ATF2 (Ser94, Ser112) Polyclonal Antibody
ATF2 단백질의 Ser94 및 Ser112 인산화 부위를 인식하는 rabbit polyclonal 항체로, Western blot 및 IHC에 적합합니다. Protein A로 정제되었으며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 세포 스트레스 신호 연구용으로 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence that includes phosphorylation sites of Serine 112 or 94 (D-L-S(p)-P-L) derived from human ATF2 and conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | Dulbecco’s PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The transcription factor ATF-2 (also known as CRE-BP1) binds to both AP-1 and CRE DNA response elements and belongs to the ATF/CREB family of leucine zipper proteins.
ATF-2 interacts with various viral oncoproteins and cellular tumor suppressors and serves as a target of the SAPK/JNK and p38 MAP kinase signaling pathways.
Cellular stress, such as genotoxic agents, inflammatory cytokines, and UV irradiation, stimulates ATF-2 transcriptional activity.
Activation occurs through phosphorylation at Thr69 and Thr71, mediated by SAPK and p38 MAPK both in vitro and in transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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