Thermo Fisher Scientific HLA-DRB3 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Positive test controls: Raji, Mouse liver, Rat liver
• Subcellular localization: Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane

Immunogen sequence:
GDTRPRFLEL RKSECHFFNG TERVRYLDRY FHNQEEFLRF DSDVGEYRAV TELGRPVAES WNSQKDLLEQ KRGRVDNYCR HNYGVGESFT VQRRVHPQVT VYPAKTQPLQ HHNLLVCSVS GFYPGSIEVR WFRNGQEEKA GVVSTGLIQN GDWTFQTLVM LETVPRSGEV YTCQVEHPSV TSALTVEWRA RSESAQSK

Target Information

Major histocompatibility complex (MHC) class II molecules destined for presentation to CD4+ helper T cells are regulated by two key events:

1. Dissociation of class II-associated invariant chain peptides (CLIP) from the antigen-binding groove in MHC II α/β dimers via MHC molecules HLA-DM and -DO.
2. Subsequent peptide antigen binding.

HLA-DM and -DO molecules accumulate in endosomal/lysosomal compartments and on B cell surfaces, regulating CLIP dissociation and promoting exogenous peptide binding to HLA class II molecules (HLA-DR, -DQ, -DP).
RFLP analysis of HLA-DM genes from rheumatoid arthritis (RA) patients suggests certain polymorphisms may contribute to RA susceptibility.

HLA-B belongs to the HLA class I heavy chain paralogs, which play a central role in presenting peptides derived from the endoplasmic reticulum lumen. HLA-B and -C can form heterodimers with β2-Microglobulin, and polymorphisms yield hundreds of alleles.

HLA and MHC antibodies are essential in Immunopeptidomics, aiding in the identification and characterization of neoantigens via high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.